What is Pseudomonas aeruginosa PAO1?

What is Pseudomonas aeruginosa PAO1?

Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen. Strain PAO1, a derivative of the original Australian PAO isolate, has been distributed worldwide to laboratories and strain collections.

How many genes are encoded by the Pseudomonas aeruginosa genome?

More than 500 regulatory genes were identified in the genome of strain PAO1 (Stover et al., 2000). The genomes of P. aeruginosa strains are larger than those of most sequenced bacteria. Within the species, the genome size varies between 5.5 and 7 Mbp (Schmidt et al., 1996; Lee et al., 2006).

How many chromosomes does Pseudomonas aeruginosa have?

In the 1970’s Booker and Loutit proposed that Pseudomonas aeruginosa strain PAO1 has two chromosomes, based on conjugational linkage studies (Booker & Loutit, 1974).

What is unique about Pseudomonas aeruginosa with respect to its genome?

Pseudomonas aeruginosa is an opportunistic bacterial pathogen able to thrive in highly diverse ecological niches and to infect compromised patients. Its genome exhibits a mosaic structure composed of a core genome into which accessory genes are inserted en bloc at specific sites.

What family does Pseudomonas aeruginosa belong to?

PseudomonadaceaePseudomonas aeruginosa / Family

What is the Gram stain morphology of Pseudomonas aeruginosa?

Pseudomonas aeruginosa is a Gram-negative rod measuring 0.5 to 0.8 µm by 1.5 to 3.0 µm. Almost all strains are motile by means of a single polar flagellum.

Why is Pseudomonas aeruginosa considered an opportunistic pathogen?

P. aeruginosa is often resistant to many classes of antibiotics and therapeutic agents, and this makes it problematic during infection as it can be difficult to treat. It is often termed an ‘opportunistic’ pathogen because it rarely infects healthy individuals.

What is the cell arrangement of Pseudomonas aeruginosa?

Pseudomonas aeruginosa PAO1

Names Pseudomonas aeruginosa PAO1
Cell arrangement Singles
Sporulation Nonsporulating
Metabolism NA
Energy source Heterotroph

How does Pseudomonas aeruginosa reproduce?

Because Pseudomonas aeruginosa is prokaryotic, and a single celled organism, it must reproduce asexually. Asexual reproduction does not involve the fusion of gametes, or genetic recombination as found in sexual reproduction. Specifically, it clones itself through the process of binary fission.

What are the characteristics of Pseudomonas aeruginosa?

Pseudomonas aeruginosa is a Gram-negative, rod-shaped, asporogenous, and monoflagellated bacterium. It has a pearlescent appearance and grape-like or tortilla-like odour. P. aeruginosa grows well at 25°C to 37°C, and its ability to grow at 42°C helps distinguish it from many other Pseudomonas species.

What order is Pseudomonas in?

PseudomonadalesPseudomonas / Order

How would you differentiate Pseudomonas aeruginosa from other members of the fluorescent Pseudomonad group?

They can both be distinguished from other pseudomonads by their negative oxidase reaction and production of non-diffusible yellow pigment. Primary culture for Pseudomonas species should be performed on blood agar and/or Pseudomonas selective agar.

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections. A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants.

What is the genome sequence of Pseudomonas aeruginosa strain AH16?

Genome sequence of Pseudomonas aeruginosa strain AH16, isolated from a patient with chronic pneumonia in China. Draft genome sequence of the Pseudomonas aeruginosa bloodstream isolate PABL056. Genome sequence of Pseudomonas aeruginosa DQ8, an efficient degrader of n-alkanes and polycyclic aromatic hydrocarbons.

How many base pairs are there in PAO1?

Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of the versatility and intrinsic drug resistance of P. aeruginosa.

What is the basis for the Pseudomonas aeruginosa genome prediction?

They are currently based on schemes at the PubMLST website (https://pubmlst.org/) developed by Keith Jolley (Jolley & Maiden 2010, BMC Bioinformatics, 11:595) that were predicted by the “mlst” software developed by Torsten Seemann (Github https://github.com/tseemann/mlst). Pseudomonas aeruginosa PAO1 chromosome, complete genome.

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