What is CFSE labeling?

What is CFSE labeling?

CFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and fluorescent microscopy. The dye is long lasting and well retained within labeled cells. The provided CFSE is sufficient for ~1000 assays.

What is CFSE proliferation assay?

The Invitrogen CellTrace CFSE kit is used to monitor distinct generations of proliferating cells by dye dilution. Live cells are covalently labeled with a very bright, stable dye. Every generation of cells appears as a different peak on a flow cytometry histogram.

How do you measure proliferation of T cells?

T-cell proliferation is usually assessed by measuring incorporation of a radioactive tracer ([3H]thymidine), which gives the total amount of DNA synthesized in a bulk culture but provides no information on the actual frequency of specific T cells.

How do you analyze cell proliferation?

There are several methods available to measure cell proliferation rates. One method is to measure the overall metabolic activity inside a cell. Several dyes are available that can permeabilize a cell and react with certain enzymes and other factors and form a colored end-product which can be easily detected.

What does CFSE stand for?

Carboxyfluorescein succinimidyl ester

Other names CFSE; Carboxyfluorescein N-succinimidyl ester
CAS Number 92557-81-8 117548-22-8 (5/6 mixture)
3D model (JSmol) Interactive image

Is CFSE toxic to cells?

CFSE can be toxic to cells and non-optimal CFSE labeling conditions can thus hamper proliferation of cells and obscure interpretation of results.

What color is CFSE?

green fluorescence
CFSE (Carboxyfluorescein succinimidyl ester) is a membrane-permeable fluorescent dye. As the dye diffuses into the cell, its succinimidyl group covalently binds to intracellular lysine residues and other free primary amines, producing green fluorescence.

What is CFSE?

The crystal field stabilization energy (CFSE) is the stability that results from placing a transition metal ion in the crystal field generated by a set of ligands.

How does Cfse staining work?

Unlike stains that label the lipid membrane of cells, CellTraceā„¢ CFSE dye easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye is designed to provide a consistent signal, even after several days in a cell culture environment.

What are cell proliferation assays?

Cell proliferation assays are designed to quantify the relative rates of cell division within such target tissues using specialized immunohistochemical staining techniques to detect proliferating cells.

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