What is BrdU incorporation?
Abstract. Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.
How does BrdU labeling work?
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. Once incorporated, these nucleoside analogs serve as cell cycle and proliferation markers that can be detected using labeled probes to identify cells that are actively proliferating.
In which phase of the cell cycle does BrdU incorporation occur?
An accurate method for detection of cell proliferation uses direct measurement of new DNA synthesis. First, the thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA in cells entering and progressing through the S (DNA synthesis) phase of the cell cycle.
What are BrdU cells?
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation.
How do you do a BrdU assay?
Prepare a 10 mM stock solution of BrdU (ab142567) by dissolving 3 mg of BrdU in 1 mL water. Dilute the 10 mM BrdU stock solution in cell culture medium to make a 10 µM BrdU labeling solution. Filter the 10 µM BrdU labeling solution through a 0.2 µm filter under sterile conditions.
What does BrdU stain for?
What kind of cells does BrdU identify?
Bromodeoxyuridine (BrdU) is a thymidine analog to which monoclonal antibodies have been developed. This methodology allows detection of endothelial cells in the S phase at the light microscopic level but, unlike autoradiography, there is no 3- to 4-week exposure period.
What kind of cells does BrdU stain for?
BrdU (Bromodeoxyuridine / 5-bromo-2′-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cells within a living animal.
Is BrdU incorporated into RNA?
BrdU incorporation is frequently used in proliferation assays to study DNA repair, sister chromatid exchange, and the cytokinetics of normal and neoplastic cells. BrU (5-Bromouridine) is a uridine analogue, same as BrdU, and is used to study RNA synthesis.
How does BrdU cause mutation?
BrdU-labelled cells in humans can be detected up to two years after BrdU infusion. Because BrdU can replace thymidine during DNA replication, it can cause mutations, and its use is therefore potentially a health hazard.