What is streptavidin PE?
PE Streptavidin is a second-step reagent useful when detecting biotinylated antibodies in indirect staining protocols. Streptavidin is a nonglycosylated biotin-binding protein that is bacterially derived. Like avidin, it binds to biotin with high affinity but displays less nonspecific binding.
What is streptavidin phycoerythrin?
Streptavidin phycoerythrin, also known as SA-PE or R-PE streptavidin, is typically used for flow cytometry, microarrays, ELISA, and other applications that require either high sensitivity or simultaneous multicolor detection.
What is the molecular weight of streptavidin?
52 kDa
It is purified to a high degree from fermentations of Streptomyces avidinii and has a molecular weight of 52 kDa (also known as ‘core’ streptavidin).
What is the size of streptavidin?
60 KDa
Streptavidin is a 60 KDa tetrameric protein with a globular subunit organization of 5 nm in size (Kuzuya et al., 2008)—a large and bulky linker compared to alternative techniques.
Why is streptavidin used?
Streptavidin is widely used in Western blotting and immunoassays conjugated to some reporter molecule, such as horseradish peroxidase. Streptavidin has also been used in the developing field of Nanobiotechnology, the use of biological molecules such as proteins or lipids to create nanoscale devices/structures.
What is the function of streptavidin?
Like Avidin, Streptavidin is thought to function as an antibiotic and has a very high affinity for biotin (Kd = 10-14 to -15M). Unlike Avidin, Streptavidin has no carbohydrate and has an acidic isoelectric point (pI = 5) that gives Streptavidin a significantly lower solubility than Avidin.
Is streptavidin an enzyme?
Enzyme Conjugated Avidin/Streptavidin Avidin and Streptavidin are offered conjugated with either alkaline phosphatase (AP) or horseradish peroxidase (HRP) in concentrate or ready-to-use formats. Use these reagents for enzyme-based detection of biotinylated macromolecules including antibodies and nucleic acids.
Where is streptavidin from?
Streptavidin. Streptavidin is a tetrameric biotin-binding protein that is isolated from Streptomyces avidinii and has a mass of 60,000 daltons.
What is streptavidin used for?
Is streptavidin a fluorescent?
In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light.
What color is streptavidin?
blue fluorescence
AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. This derivative excites in the ultraviolet (350 nm) and emits in the visible (450 nm) producing an intense blue fluorescence.
What is the difference between Neutravidin and streptavidin?
Avidin, streptavidin, and neutravidin are functional and structural analogues that bind to biotin with extremely high affinity. Avidin is derived from eggs of oviparous vertebrates17, while streptavidin is derived from Streptomyces avidinii. Neutravidin is a chemically modified avidin without glycosylation.
What is the molecular weight of streptavidin PE?
Streptavidin-Phycoerythrin (PE) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE is 360 kD and Streptavidin alone is 52 kD.
What is streptavidin-phycoerythrin (PE)?
Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm. Product Details Technical data sheet Product Details
What is streptavidin and how is it made?
Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
How is the concentration of streptavidin tested for quality control?
This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE conjugation.