What is ITS1 and ITS4?
The ITS1-ITS4 primer pair was used to amplify the intervening 5.8S rDNA and the adjacent ITS1 and ITS2 regions, and the ITS3-ITS4 primer pair was used to amplify a large portion of the 5.8S rDNA and the adjacent ITS2 region (Fig.
What is its in PCR?
The Internal Transcribed Spacer (ITS) regions of fungal ribosomal DNA (rDNA) are highly variable sequences of great importance in distinguishing fungal species by PCR analysis.
What is its region in fungi?
The ITS region is the most widely sequenced DNA region in molecular ecology of fungi and has been recommended as the universal fungal barcode sequence. It has typically been most useful for molecular systematics at the species to genus level, and even within species (e.g., to identify geographic races).
Is ITS1 forward primer?
Diagram of primer locations in the ribosomal cassette consisting of SSU, ITS1, 5.8S, ITS2, and LSU rDNA. Primers are positioned above (forward primers) or below (reverse) their sequence positions. ITS1, ITS2, ITS3, and ITS4 from White et al.
What is the difference between 18S and its?
Similar to 18S rRNA, ITS is often used in metagenomic analysis. However, 18S rRNA is mainly used for high resolution taxonomic studies of fungi, while the ITS region is mainly used for fungal diversity studies as a fungal barcode marker.
What is the difference between ITS1 and ITS2?
The key difference between ITS1 and ITS2 is that ITS1 is a spacer DNA located between 18S and 5.8S rRNA genes in eukaryotes, while ITS2 is a spacer DNA located between 5.8S and 28S rRNA genes in eukaryotes.
How many ITS regions are there in fungi?
Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium.
What is ITS1 primer?
The internal transcribed spacer 1 (ITS1) region of the eukaryotic ribosomal cluster has features allowing for wide taxonomic coverage and has been recognized as a suitable barcode region for species-level identification of fungal organisms. We developed custom ITS1 primer sets using iterative alignment refinement.
What is 18S rDNA sequencing?
18S rRNA gene sequencing is commonly used to identify, classify, and quantify microbes within complex biological mixtures such as samples collected from the environment and gut.
What are 16s and 18S rRNA?
16s rRNA is present in the small subunit of prokaryotic ribosomes as well as mitochondrial ribosomes in eukaryotes. 18s is the homologous small subunit rRNA of eukaryotes.
Which is an advantage of working with short DNA fragments?
Which is an advantage of working with short DNA fragments? They are more stable and less likely to break apart.