What is DMEM Gibco?
Gibco Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What are the significant features of DMEM media?
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
What is added to DMEM?
Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media.
What is the difference between DMEM and RPMI?
DMEM (low glucose) contains a lower concentration of glucose (1 g/L) than RPMI 1640 (2 g/L). In addition, DMEM has a higher concentration in calcium (1.8 mM) and a lower concentration in phosphate (1 mM) than RPMI 1640 which contains 0.8 mM of calcium and 5 mM of phosphate.
What is Gibco?
Gibco stands for the highest quality sera, media and research products for over fifty years. Gibco media, supplements, cells, and cell culture reagents are designed to deliver reproducibility and performance for results you can count on every day.
Why is DMEM used in cell culture?
DMEM is a liquid serum-free media and a variant of BME, or Basal Medium Eagle, modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM. DMEM’s composition makes it an ideal supplement for most cell culture media applications.
What is in DMEM media?
Dulbecco’s Modified Eagle’s Medium (DMEM) is modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. Contains 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.
What is the difference between DMEM and MEM?
DMEM is richer in a variety of nutrients than MEM and is a very good choice for many cell types. Cells will grow faster and deplete the medium slower in DMEM. You should also consider Opti-MEM which, in my experience, is even better than DMEM. HEK cells will grow fine and very similar at 5-10% FBS in both MEM and DMEM.
What is Gibco cell culture?
Gibco Cell Culture Basics provides an introduction to cell culture, covering topics such as laboratory setup, safety, and aseptic techniques. You’ll also find basic cell culture techniques and methods for passaging, freezing, and thawing cultured cells.
Where is Gibco based?
Waltham, Massachusetts
Invitrogen
| Type | Subsidiary |
|---|---|
| Headquarters | Waltham, Massachusetts, USA |
| Key people | Marc N. Casper (President & CEO) |
| Parent | Thermo Fisher Scientific |
What is DMEM made of?