What is APC in flow cytometry?
APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.
What is APC and PE?
Phycoerythrin (PE) and allophycocyanin (APC) are members of a protein family called phycobiliproteins. This family of proteins is present in photosynthetic organisms, such as algae and cyanobacteria. In nature, they typically act as accessory proteins to chlorophyll pigments in photosynthesis.
What does APC stain for?
APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.
Does PE and APC overlap?
Furthermore, PE (and PE-Texas Red to an even greater extent) does overlap significantly with APC.
Does PE and FITC overlap?
Relative contribution. In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons.
Is FITC same as 488?
High-performance alternatives to fluorescein The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.
Does FITC overlap APC?
5 or APC). 2)Move CD62L to a detector that is still relatively bright, but does not overlap with FITC (such as APC or PE-Cy5). Note that in this example, only the CD8+ cells are highly stained in the FITC detector, so only CD8+ cells contribute to data spread in the PE detector.
What is the purpose of FACS?
Fluorescence-activated cell sorting (FACS) is a technique to purify specific cell populations based on phenotypes detected by flow cytometry. This method enables researchers to better understand the characteristics of a single cell population without the influence of other cells.
What laser is PE?
PE is excitable by both the 488 nm laser and 561 nm laser, therefore PE and its tandem dyes appear here and on the 488 nm excitable fluorophores web page. As PE is maximally excited at 546 nm (compared to 496 nm using the 488 nm laser), where possible it is advisable to use the 561 nm laser to get the maximal signal.