What are transfected cells?
Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection.
What is lipofectamine transfection?
Lipofectamine® Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine® Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with many cell lines.
Can you transfect stem cells?
Transfection is used to reprogram somatic cells to induced pluripotent stem cells (iPSCs), and to engineer stem cells for therapeutic or research purposes.
How do you transfect IPS cells?
2. Transfection of human iPSCs with GeneJuice
- Prepare 100 μl KO-DMEM/F12 in a sterile 1.5 ml eppendorf tube.
- Add 4 μg plasmid DNA.
- Add GeneJuice-DNA mixture to the cells and swirl the plate.
- Incubate the cells at 37°C overnight.
- Monitor for transfection efficiency the next day.
Where is HEK293 from?
HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
What can you do with transfected cells?
The two main purposes of transfection are to produce recombinant proteins, or to specifically enhance or inhibit gene expression in transfected cells.
What is optimem?
Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
What lipid is lipofectamine?
Lipid-mediated transfection relies on cationic lipids, such as lipofectamine, which facilitate DNA and mRNA delivery into cells. The cationic lipids form a unilamellar liposomal structure with a positive surface charge and fuse with nucleic acids to form a transfection complex.
Why are stem cells hard to transfect?
Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with plasmid DNA. This is mainly due to their fragility and slow-dividing rates.
What is the difference between electroporation and Nucleofection?
With its superior transfection performance, Nucleofection offers various advantages over traditional electroporation methods: High transfection efficiencies of up to 90% for plasmid DNA and 99% for oligonucleotides, like siRNA. Excellent preservation of the physiological status and viability of transfected cells.
What is HEK293 used for?
HEK293 are frequently used for heterologous expression of cell membrane receptors and ion channels, and many studies have utilized this cell line to investigate the signalling pathways of G protein-coupled receptors (GPCRs) which are implicated in disease.
Why is it called HEK293?
From all the experiments Graham and van der Eb conducted, only a single clone survived this daunting endeavor. Unfortunately, this process was not very efficient and the name of the cell line – HEK293 – stems from a numbering system, making this the 293rd experiment.
What is a lipostem injection?
Lipostem is the painless injection of your own fat together with your platelet rich plasma into your fat pad done under ultrasound guidance. What Is Fat Pad Atrophy And How Do People Get It?
Can lipostem help with fat pad atrophy?
Many patients have come to use suffering from fat pad atrophy. In the past, we had no treatment for them. Now we have an excellent option: Lipostem. Lipostem is the painless injection of your own fat together with your platelet rich plasma into your fat pad done under ultrasound guidance.
What is Invitrogen Lipofectamine stem transfection reagent?
Designed for dramatically improved transfection efficiency, Invitrogen Lipofectamine Stem Transfection Reagent offers a simple, robust and reproducible method for delivering DNA, RNA and RNP such as Cas9/gRNA complexes into a wide range of stem cells, including pluripotent stem cells, neural stem cells and mesenchymal stem cells (Figure 2).
Is Lipofectamine stem reagent compatible with my culture system?
While Lipofectamine Stem reagent is compatible with most culture systems, components in some stem cell media can interfere with liposomal-based transfection.