Is Eppendorf tube same as microcentrifuge tube?
The microcentrifuge tube, often referred to as the Eppendorf Tube, is essential to any lab that handles small volumes of liquid. In life science and biological labs, these little plastic tubes are used for all sorts of applications from sample storage to running reactions and spinning down or separating samples.
What is the function of microcentrifuge tubes?
Microcentrifuge tubes or microfuge tubes are small, cylindrical plastic containers with conical bottoms, typically with an integral snap cap. They are used in molecular biology and biochemistry to store and centrifuge small amounts of liquid.
Can you centrifuge tubes without caps?
Use these micro centrifuge tubes for a variety of applications. Fisherbrand™ Polypropylene Microcentrifuge Tubes without Cap are ideal for handling needs where a snap cap can interfere.
Are Eppendorf tubes autoclavable?
Can they be autoclaved? yes, they can be autoclaved. Eppendorf tubes aren’t that expensive.
Can you autoclave Eppendorf tubes?
Autoclave the treated tubes to remove the DEPC. Not removing DEPC will strongly influence your downstream reactions. 3. Place the autoclaved tubes in the oven at 65*C and leave them to properly dry.
Do Eppendorf tubes melt?
Never it can happen. You can check it by yourself. During routine procedures for DNA extraction, phenol-chloroform is used which has never put an negative effect on the Eppendorf tube as far as my experience is concerned. This will answer your question, if you are using Eppendorf made by polypropylene (PP).
Are Eppendorf tubes sterile?
Eppendorf Safe-Lock 1.5 mL snap-cap tubes for microcentrifuge style Bullet Blenders®. Sterile, RNase & DNase free.
Are Eppendorf tubes airtight?
Water does sublimate at -80°C. Eppendorf tubes (and for that matter cryogenic storage vials) are not gas tight at -80°C and parafilm seems to do little or nothing to prevent to exchange of gases in the head space of Eppendorf tubes.
How do you sterilize microcentrifuge tubes?
Popular Answers (1)
- Incubate tubes with 0.1% diethylpyrocarbonate (DEPC) treated water for 10-20 minutes, RT.
- Autoclave the treated tubes to remove the DEPC. Not removing DEPC will strongly influence your downstream reactions.
- Place the autoclaved tubes in the oven at 65*C and leave them to properly dry.
Do PCR tubes need to be sterile?
You do not need to autoclave PCR tubes, and it may be worse, not better to do so. Sterile is not the same as clean. You care about contaminating DNA (primed by your primers) not a few cells which won’t replicate at 95C. An autoclave can be a source, not a cure for this contamination.
What is the size of the Eppendorf tubes?
Eppendorf Tubes ® 5.0 mL with screw cap , 5.0 mL, Eppendorf Quality™, colorless, 200 tubes (2 bags × 100 tubes). Replaces order no. 0030119517. Catalog No. 0030122305 Ergonomic operation: Snap cap – simple, practical and ergonomic singlehand operation. Screw cap – flattened and grooved sides provide optimal grip and user-friendly handling.
Which Eppendorf microtube is best for high speed centrifugation?
Eppendorf microtubes ® 5 mL are suitable for high speed centrifugation. Eppendorf microtube ® rack with 5 mL and 1.5 mL tubes. Eppendorf offer a comprehensive range of DNA LoBind ® accesories and are the perfect option for working with precious DNA samples!
What are the test conditions for the Eppendorf tube rack?
(Test conditions: 45° fixed-angle rotor at 40 °C, with aqueous salt solution (density 1.0 g/mL) and 90 mins). The mechanical loading capacity of the tubes is reduced by the use of organic solvents. Eppendorf Tube Racks and Eppendorf Cuvett…
Why amber colored Eppendorf tubes?
The amber colored Eppendorf Tubes ® 5.0 mL with screw cap combine effective protection of your sensitive samples from energy-rich light in the lower wavelength range with a high degree of transparency.